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In recent years, there have been considerable developments in techniques for the investigation and utilization of enzymes. Enzymes can build up or break down other molecules. The molecules they act on are called substrates. Enzymes are catalysts-chemicals that hasten a chemical reaction without undergoing any change themselves. Enzyme Technology is one the most promising disciplines in modern biotechnology. Most of the traditional chemical-based processes are being replaced by enzyme-based processes. In this text, the applications of a wide variety of enzymes are highlighted.